Comparison of a Multiplex and 5' Nuclease PCR Assays for the Rapid Detection of Pathogenic Yersinia enterocolitica in Swine and Pork Products
نویسندگان
چکیده
Bacteriological culture methods were compared with PCR based protocols (multiplex PCR and TaqMan assay) for the rapid detection of pathogenic Yersinia enterocolitica (YE) in market weight hogs and pork products. The prevalence of YE was compared in lairaged hogs (n=150) versus hogs transported directly to the farm (n=150). By bacteriological culture, YE was not detected in any of the hog tissues tested but was detected by multiplex PCR and TaqMan assay. We also screened ground pork and chitterlings for the presence of YE. By standard culture, YE was detected in chitterlings (8%). By multiplex PCR, YE was identified in ground pork (10%) and chitterlings (27%). TaqMan assay identified YE in ground pork (44%) and chitterlings (79%).
منابع مشابه
Comparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of Yersinia enterocolitica in swine and pork products.
Bacteriological culture was compared with multiplex and fluorogenic (TaqMan) polymerase chain reaction (PCR) assays for the detection of attachment invasion locus (ail)-bearing Yersinia enterocolitica in market weight swine, chitterlings, and ground pork. The TaqMan assay detected 1 pg of purified Y. enterocolitica DNA, whereas conventional gel-based PCR detected I ng of the same. The presence ...
متن کاملIdentification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and pulsed-field gel electrophoresis.
Approximately 550 to 600 yersiniosis patients are reported annually in Sweden. Although pigs are thought to be the main reservoir of food-borne pathogenic Yersinia enterocolitica, the role of pork meat as a vehicle for transmission to humans is still unclear. Pork meat collected from refrigerators and local shops frequented by yersiniosis patients (n=48) were examined for the presence of pathog...
متن کاملDetection of Yersinia enterocolitica in Pigs and Pork Products
Introduction Yersinia enterocolitica is a zoonotic organism affecting both humans and livestock. In humans, Y. enterocolitica infection can result in severe abdominal pain, which mimics appendicitis. Swine have been implicated as the principal animal reservoir for human pathogenic Y. enterocolitica. Infections in pigs may localize in the tonsils (3). Several outbreaks of yersiniosis have involv...
متن کاملComparison of ELISA and PCR Assays for Detection of Pork Adulteration in Halal-Labelled Beef Products
Background: Food adulteration with pork in processed beef products is one of the most serious issues in a food sector in a Muslim-majority country since it is related to religious food ethics regarding the halal products. The goal of this research is to test the suitability of ingredients in beef floss and its Halal by knowing the presence of pork DNA and protein in those products. Methods: Me...
متن کاملPrevalence of pathogenic Yersinia enterocolitica strains in pigs in the United States.
Yersinia enterocolitica is considered an important food-borne pathogen impacting the pork production and processing industry in the United States. Since this bacterium is a commensal of swine, the primary goal of this study was to determine the prevalence of pathogenic Y. enterocolitica in pigs in the United States using feces as the sample source. A total of 2,793 fecal samples were tested for...
متن کامل